A nurse is caring for a patient with a confirmed Gram-negative bacterial infection. The healthcare provider has prescribed an antibiotic regimen. Which of the following considerations is most important when treating Gram-negative organisms compared to Gram-positive organisms?

A. Gram-negative bacteria have a thinner peptidoglycan layer compared to Gram-positive bacteria. The thicker peptidoglycan of Gram-positive bacteria is more easily targeted by certain antibiotics like penicillin. B. Gram-negative bacteria have an outer membrane with lipopolysaccharides, which acts as a barrier to many antibiotics. This structural complexity often necessitates the use of antibiotics that can penetrate the outer membrane or have mechanisms to bypass this barrier. C. Gram-negative bacteria are more prone to developing antibiotic resistance due to their outer membrane and efflux pumps. This complicates treatment decisions rather than simplifying them, requiring vigilance in antibiotic selection and monitoring. D. While Gram-negative bacteria do have a thinner peptidoglycan layer, their outer membrane protects them from many antibiotics, so they are not inherently more susceptible. Treatment must consider this additional barrier to ensure efficacy.