The pregnancy test for a 15-year-old female is positive. Which test(s) are NOT usually performed during the first trimester of pregnancy?

Rationale: A. Blood type, Rh factor, and antibody screens are routinely performed in the first trimester to identify potential incompatibilities and plan appropriate management. B. Screening for STDs, including HIV, syphilis, chlamydia, and gonorrhea, is recommended early in pregnancy to ensure maternal and fetal health. C. A purified protein derivative (PPD) test for tuberculosis may be performed in the first trimester if the patient is at risk. D. Alpha-fetoprotein (AFP) screening is typically performed during the second trimester (around 15–20 weeks gestation) to assess for neural tube defects and other fetal abnormalities. It is not a routine first-trimester test.